Issue 5, 2011
From the journal:

Chemical Science

Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells†

Yue Pan,‡a   Marcus J. C. Long,‡b   Xinming Li,a   Junfeng Shi,a   Lizbeth Hedstromac  and  Bing Xu*a  

* Corresponding authors

a Department of Chemistry, Brandeis University, 415 South Street, Waltham, MA, USA
E-mail: bxu@brandeis.edu
Fax: +7817362516
Tel: +781-736-5201

b Graduate Program in Biochemistry and Biophysics, Brandeis University, 415 South Street, Waltham, MA, USA

c Department of Biology, Brandeis University, 415 South Street, Waltham, MA, USA

Abstract

Iron oxide-based magnetic nanoparticles (MNP) surface-decorated with glutathione (GSH) via a dopamine anchor bind to human α1-glutathione S-transferase (GST) with high affinity and specificity and are able to separate GST fusion proteins from cell lysates. Both the purified GST and the protein of interest (POI) preserve their innate properties. The conjugate of MNP and the GST fusion protein also enables magnetic manipulation of cells.

Graphical abstract: Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

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Article information

DOI
https://doi.org/10.1039/C1SC00030F
Article type
Edge Article
Submitted
20 Jan 2011
Accepted
17 Feb 2011
First published
03 Mar 2011

Chem. Sci., 2011,2, 945-948

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Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

Y. Pan, M. J. C. Long, X. Li, J. Shi, L. Hedstrom and B. Xu, Chem. Sci., 2011, 2, 945 DOI: 10.1039/C1SC00030F

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