Issue 6, 2013

Antibody orientation on biosensor surfaces: a minireview

Abstract

Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

Graphical abstract: Antibody orientation on biosensor surfaces: a minireview

Article information

Article type
Minireview
Submitted
01 Dec 2012
Accepted
06 Jan 2013
First published
22 Jan 2013
This article is Open Access
Creative Commons BY license

Analyst, 2013,138, 1619-1627

Antibody orientation on biosensor surfaces: a minireview

A. K. Trilling, J. Beekwilder and H. Zuilhof, Analyst, 2013, 138, 1619 DOI: 10.1039/C2AN36787D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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