Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate

Keiichi Ohtsuka; Shinobu Sato; Yusuke Sato; Kojiro Sota; Shinsuke Ohzawa; Tomoki Matsuda; Kiwamu Takemoto; Nobutoki Takamune; Bernard Juskowiak; Takeharu Nagai; Shigeori Takenaka

doi:10.1039/C2CC30536D

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate†

Keiichi Ohtsuka,^a Shinobu Sato,^a Yusuke Sato,^a Kojiro Sota,^a Shinsuke Ohzawa,^a Tomoki Matsuda,^b Kiwamu Takemoto,^b Nobutoki Takamune,^c Bernard Juskowiak,^d Takeharu Nagai^b and Shigeori Takenaka*^a

Author affiliations

* Corresponding authors

^a Department of Applied Chemistry, Graduate School of Engineering, Kyushu Institute of Technology, Fukuoka 804-8550, Japan
E-mail: shige@che.kyutech.ac.jp
Fax: +81-93-884-3322

^b Department of Biomolecular Science and Engineering, Institute of Scientific and Industrial Research, Osaka University, Osaka 560-0047, Japan

^c Department of Pharmaceutical Biochemistry, Kumamoto University, Kumamoto 862-0973, Japan

^d Faculty of Chemistry, A. Mickiewicz University, Poznan 60-780, Poland

Abstract

When a biotinylated FRET probe based on a peptide–thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K⁺ concentration changes in the cell.

Chemical Communications

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate†

Abstract

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Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate

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