Issue 44, 2012
From the journal:

Chemical Communications

A general method for detecting protease activity viagelation and its application to artificial clotting†

Steven C. Bremmer,a   Jing Chen,b   Anne J. McNeil*b  and  Matthew B. Soellner*a  

* Corresponding authors

a Departments of Medicinal Chemistry and Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan, USA
E-mail: soellner@umich.edu

b Department of Chemistry and Macromolecular Science and Engineering Program, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan, USA
E-mail: ajmcneil@umich.edu

Abstract

A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.

Graphical abstract: A general method for detecting protease activity via gelation and its application to artificial clotting

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC31537H
Article type
Communication
Submitted
29 Feb 2012
Accepted
29 Mar 2012
First published
30 Mar 2012

Chem. Commun., 2012,48, 5482-5484

Permissions

Request permissions

A general method for detecting protease activity via gelation and its application to artificial clotting

S. C. Bremmer, J. Chen, A. J. McNeil and M. B. Soellner, Chem. Commun., 2012, 48, 5482 DOI: 10.1039/C2CC31537H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements