A target-activated autocatalytic DNAzyme amplification strategy for the assay of base excision repair enzyme activity

Abstract

Based on a target-activated autocatalytic DNAzyme amplification strategy, novel fluorescence sensing platforms were constructed for highly sensitive and selective assay of base excision repair enzyme activity. By using a rolling circle amplification (RCA)-coupled amplification cascade, an extremely low detection limit (0.002 U mL⁻¹) was achieved.