Issue 7, 2012

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

Abstract

We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20 000 mammalian cells s−1 with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s−1 or 3000 beads s−1. Fast switching (30 μs) and a small perturbation volume (∼90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.

Graphical abstract: Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

Supplementary files

Article information

Article type
Paper
Submitted
08 Nov 2011
Accepted
09 Feb 2012
First published
15 Feb 2012

Lab Chip, 2012,12, 1378-1383

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

T. Wu, Y. Chen, S. Park, J. Hong, T. Teslaa, J. F. Zhong, D. Di Carlo, M. A. Teitell and P. Chiou, Lab Chip, 2012, 12, 1378 DOI: 10.1039/C2LC21084C

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