Issue 6, 2012
From the journal:

Lab on a Chip

Application of click chemistry to the production of DNA microarrays†

Barbara Uszczyńska,a   Tomasz Ratajczak,a   Emilia Frydrych,b   Hieronim Maciejewski,bc   Marek Figlerowicz,ad   Wojciech T. Markiewicza  and  Marcin K. Chmielewski*a  

* Corresponding authors

a Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, Poznań, Poland
E-mail: maro@ibch.poznan.pl

b Poznań Science and Technology Park, A. Mickiewicz University Foundation, Rubież 46, Poznań, Poland
E-mail: maciejm@amu.edu.pl
Fax: +48618520532
Tel: +48618528503

c Faculty of Chemistry, A. Mickiewicz University, Grunwaldzka 6, Poznań, Poland

d Institute of Computing Science, Poznań University of Technology, Piotrowo 3A, Poznań, Poland

Abstract

The copper-catalyzed alkyne–azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5′-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.

Graphical abstract: Application of click chemistry to the production of DNA microarrays

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Article information

DOI
https://doi.org/10.1039/C2LC21096G
Article type
Paper
Submitted
10 Nov 2011
Accepted
22 Dec 2011
First published
06 Jan 2012

Lab Chip, 2012,12, 1151-1156

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Application of click chemistry to the production of DNA microarrays

B. Uszczyńska, T. Ratajczak, E. Frydrych, H. Maciejewski, M. Figlerowicz, W. T. Markiewicz and M. K. Chmielewski, Lab Chip, 2012, 12, 1151 DOI: 10.1039/C2LC21096G

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