Issue 22, 2012
From the journal:

Lab on a Chip

Single-cell electroporation using a multifunctional pipette†

Alar Ainla,a   Shijun Xu,a   Nicolas Sanchez,b   Gavin D. M. Jeffriesa  and  Aldo Jesorka*a  

* Corresponding authors

a Department of Chemical and Biological Engineering, Chalmers University of Technology, S-41296 Göteborg, Sweden

b Exploratory Unit, Sanofi-Aventis R&D, Montpellier, France

Abstract

We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.

Graphical abstract: Single-cell electroporation using a multifunctional pipette

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Article information

DOI
https://doi.org/10.1039/C2LC40563F
Article type
Communication
Submitted
16 May 2012
Accepted
20 Jun 2012
First published
22 Jun 2012

Lab Chip, 2012,12, 4605-4609

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Single-cell electroporation using a multifunctional pipette

A. Ainla, S. Xu, N. Sanchez, G. D. M. Jeffries and A. Jesorka, Lab Chip, 2012, 12, 4605 DOI: 10.1039/C2LC40563F

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