Issue 24, 2012

A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing

Abstract

We have developed a quantitative immunoassay chip targeting point-of-care testing. To implement a lateral flow immunoassay, a glass fiber sheet was chosen as the material for the microfluidic channel in which the negative charge on the fiber surfaces efficiently generates the electroosmotic flow (EOF). The EOF, in turn, allows controllable bound/free separation of antigen/antibody interactions on the chip and enables precise determination of the antigen concentration. In addition, the defined size of the porous matrix was suitable for the filtration of undesired large particles. We confirmed the linear relationship between the concentration of analyte and the resulting fluorescence intensity from the immunoassay of two model analytes, C-reactive protein (CRP) and insulin, demonstrating that analyte concentration was quantitatively determined within the developed chip in 20 min. The limits of detection were 8.5 ng mL−1 and 17 ng mL−1 for CRP and insulin, respectively.

Graphical abstract: A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing

Supplementary files

Article information

Article type
Paper
Submitted
30 Aug 2012
Accepted
02 Oct 2012
First published
03 Oct 2012

Lab Chip, 2012,12, 5155-5159

A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing

Y. Oyama, T. Osaki, K. Kamiya, R. Kawano, T. Honjoh, H. Shibata, T. Ide and S. Takeuchi, Lab Chip, 2012, 12, 5155 DOI: 10.1039/C2LC40994A

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