Issue 5, 2012

A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen

Abstract

This paper presents a novel sensor to detect proteolytically active prostate-specific antigen (PSA) by assembling a purpose-designed FITC-labeled peptide with graphene oxide (GO). The fluorescence of the dye-labeled peptide was quenched in the presence of GO. Reaction of the sensor with PSA cleaves the peptide, leading to the release of the dye moiety and a great increase in fluorescence intensity in a dose- and time-dependent manner, and PSA can be quantified accordingly. This approach is simple compared to existing methods since the GO-peptide-based sensor is easily assembled and detection can be achieved without the involvement of complicated procedures. Moreover, the applicability of the method has been demonstrated by detecting PSA in spiked urine samples.

Graphical abstract: A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen

Article information

Article type
Paper
Submitted
21 Sep 2011
Accepted
10 Jan 2012
First published
14 Feb 2012

Mol. BioSyst., 2012,8, 1441-1445

A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen

T. Feng, D. Feng, W. Shi, X. Li and H. Ma, Mol. BioSyst., 2012, 8, 1441 DOI: 10.1039/C2MB05379A

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