Issue 15, 2012
From the journal:

Nanoscale

Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method

Jozef Adamcik,a   Cecile Lara,a   Ivan Usov,a   Jae Sun Jeong,b   Francesco S. Ruggeri,b   Giovanni Dietler,b   Hilal A. Lashuel,c   Ian W. Hamleyd  and  Raffaele Mezzenga*a  

* Corresponding authors

a ETH Zurich, Food & Soft Materials Science, Institute of Food, Nutrition & Health, Schmelzbergstrasse 9, LFO E23, 8092 Zurich, Switzerland
E-mail: raffaele.mezzenga@hest.ethz.ch
Fax: +41 44 632 16 03
Tel: +41 44 632 91 40

b Laboratory of Physics of Living Matter, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland

c Laboratory of Molecular and Chemical Biology of Neurodegeneration, Brain Mind Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland

d Dept of Chemistry, University of Reading, Whiteknights Reading, UK

Abstract

We report the investigation of the mechanical properties of different types of amyloid fibrils by the peak force quantitative nanomechanical (PF-QNM) technique. We demonstrate that this technique correctly measures the Young's modulus independent of the polymorphic state and the cross-sectional structural details of the fibrils, and we show that values for amyloid fibrils assembled from heptapeptides, α-synuclein, Aβ(1–42), insulin, β-lactoglobulin, lysozyme, ovalbumin, Tau protein and bovine serum albumin all fall in the range of 2–4 GPa.

Graphical abstract: Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method

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Article information

DOI
https://doi.org/10.1039/C2NR30768E
Article type
Communication
Submitted
30 Mar 2012
Accepted
09 May 2012
First published
11 May 2012

Nanoscale, 2012,4, 4426-4429

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Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method

J. Adamcik, C. Lara, I. Usov, J. S. Jeong, F. S. Ruggeri, G. Dietler, H. A. Lashuel, I. W. Hamley and R. Mezzenga, Nanoscale, 2012, 4, 4426 DOI: 10.1039/C2NR30768E

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