Issue 1, 2013

Bioorthogonal reactions challenged: DNA templated native chemical ligation during PCR

Abstract

DNA directed chemistry is commonly performed by using nanomolar amounts of DNA templates. Herein we introduce a method that allows the use of attomolar template loads. A DNA templated native chemical ligation yields to the covalent fixation of two fluorophores while the template is being produced during polymerase chain reaction.

Graphical abstract: Bioorthogonal reactions challenged: DNA templated native chemical ligation during PCR

Supplementary files

Article information

Article type
Edge Article
Submitted
13 Jul 2012
Accepted
03 Oct 2012
First published
03 Oct 2012

Chem. Sci., 2013,4, 432-436

Bioorthogonal reactions challenged: DNA templated native chemical ligation during PCR

A. Roloff and O. Seitz, Chem. Sci., 2013, 4, 432 DOI: 10.1039/C2SC20961F

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