Use of pyridinium and flavin derivatives for recycling of catalystic amounts of NAD+ during preparative-scale horse liver alchohol dehydrogenase-catalysed oxidations of alcohols

Abstract

Use of pyridinium salts or flavin derivatives as hydrogen acceptors has provided the first practical procedure for in situ regeneration of catalytic amounts of coenzyme during preparative-scale enzymic oxidations of alcohols with up to 35-fold turnovers of NAD⁺ being achieved for the horse liver alcohol dehydrogenase-catalysed oxidation of the representative substrate cyclohexanol.