Issue 21, 1987

Cephalosporin C biosynthesis; a branched pathway sensitive to a kinetic isotope effect

Abstract

Incubation of [3-2H] penicillin N with preparations of deacetoxycephalosporin C/deacetylcephalosporin C synthetase activity from Cephalosporium acremonium CO 728 gave, along with the normal product deacetoxycephalosporin C, another β-lactam metabolite namely 7β-[(R)-5-amino-5-carboxypentanoyl]-3β-hydroxy-3α-methyl[4-2H)-cepham-4α-carboxylic acid. This material arises as a result of a deuterium isotope effect on a branched pathway in the enzymic mechanism. The 3β-hydroxy group in this substance arises from molecular oxygen.

Article information

Article type
Paper

J. Chem. Soc., Chem. Commun., 1987, 1651-1654

Cephalosporin C biosynthesis; a branched pathway sensitive to a kinetic isotope effect

J. E. Baldwin, R. M. Adlington, R. T. Aplin, N. P. Crouch, G. Knight and C. J. Schofield, J. Chem. Soc., Chem. Commun., 1987, 1651 DOI: 10.1039/C39870001651

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements