Issue 9, 2013

A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I

Abstract

A fully automated point-of-care testing (POCT) system with a surface acoustic wave (SAW) immunosensor was developed for rapid and sensitive detection of cardiac troponin I (cTnI) in body fluid (plasma and whole blood). The assay, based on gold nanoparticle sandwich immunoassay and subsequent gold staining, was performed on the SAW immunosensor packaged inside a disposable microfluidic cartridge. The entire fluidic process, including plasma separation, reagent transport, metering, and mixing, was carried out by controlling the centrifugal force acting on the rotating cartridge and laser-irradiated ferrowax microvalves. On investigation of sensor response to various cTnI concentrations, the system exhibited a high performance with a detection limit of 6.7 pg mL−1, and the coefficient of variation was less than 10% over the entire test range (10 pg mL−1 to 25 ng mL−1). On comparing this POCT system with a clinically utilized system in a physical laboratory (Centaur® XP; Siemens), a correlation coefficient of 0.998 was found, validating the diagnostic capability of the SAW immunosensor.

Graphical abstract: A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I

Supplementary files

Article information

Article type
Paper
Submitted
24 Jan 2013
Accepted
15 Feb 2013
First published
15 Feb 2013

Analyst, 2013,138, 2558-2566

A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I

W. Lee, J. Jung, Y. K. Hahn, S. K. Kim, Y. Lee, J. Lee, T. Lee, J. Park, H. Seo, J. N. Lee, J. H. Oh, Y. Choi and S. S. Lee, Analyst, 2013, 138, 2558 DOI: 10.1039/C3AN00182B

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