Issue 12, 2013
From the journal:

Analyst

Mepanipyrimhaptens and antibodies with nanomolar affinity†

Francesc A. Esteve-Turrillas,‡a   Josep V. Mercader,‡a   Consuelo Agulló,b   Antonio Abad-Somovillab  and  Antonio Abad-Fuentes*a  

* Corresponding authors

a Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA–CSI C), Agustí Escardino 7, 46980 Paterna, València, Spain
E-mail: aabad@iata.csic.es

b Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain
E-mail: antonio.abad@uv.es

Abstract

Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC50 = 3 nM).

Graphical abstract: Mepanipyrim haptens and antibodies with nanomolar affinity

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3AN00228D
Article type
Communication
Submitted
30 Jan 2013
Accepted
19 Apr 2013
First published
23 Apr 2013

Analyst, 2013,138, 3360-3364

Permissions

Request permissions

Mepanipyrim haptens and antibodies with nanomolar affinity

F. A. Esteve-Turrillas, J. V. Mercader, C. Agulló, A. Abad-Somovilla and A. Abad-Fuentes, Analyst, 2013, 138, 3360 DOI: 10.1039/C3AN00228D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements