Issue 13, 2013

An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

Abstract

To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.

Graphical abstract: An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

Supplementary files

Article information

Article type
Paper
Submitted
01 Feb 2013
Accepted
06 Apr 2013
First published
24 Apr 2013

Analyst, 2013,138, 3660-3666

An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification

M. Ouyang, R. Mohan, Y. Lu, T. Liu, K. E. Mach, M. L. Y. Sin, M. McComb, J. Joshi, V. Gau, P. K. Wong and J. C. Liao, Analyst, 2013, 138, 3660 DOI: 10.1039/C3AN00259D

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