Issue 21, 2013

A multi-parametric microarray for protein profiling: simultaneous analysis of 8 different cytochromesvia differentially element tagged antibodies and laser ablation ICP-MS

Abstract

The paper presents a new multi-parametric protein microarray embracing the multi-analyte capabilities of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The combination of high throughput reverse phase protein microarrays with element tagged antibodies and LA-ICP-MS makes it possible to detect and quantify many proteins or biomarkers in multiple samples simultaneously. A proof of concept experiment is performed for the analysis of cytochromes particularly of cytochrome P450 enzymes, which play an important role in the metabolism of xenobiotics such as toxicants and drugs. With the aid of the LA-ICP-MS based multi-parametric reverse phase protein microarray it was possible to analyse 8 cytochromes in 14 different proteomes in one run. The methodology shows excellent detection limits in the lower amol range and a very good linearity of R2 ≥ 0.9996 which is a prerequisite for the development of further quantification strategies.

Graphical abstract: A multi-parametric microarray for protein profiling: simultaneous analysis of 8 different cytochromes via differentially element tagged antibodies and laser ablation ICP-MS

Supplementary files

Article information

Article type
Paper
Submitted
07 Mar 2013
Accepted
02 May 2013
First published
02 May 2013

Analyst, 2013,138, 6309-6315

A multi-parametric microarray for protein profiling: simultaneous analysis of 8 different cytochromes via differentially element tagged antibodies and laser ablation ICP-MS

L. Waentig, S. Techritz, N. Jakubowski and P. H. Roos, Analyst, 2013, 138, 6309 DOI: 10.1039/C3AN00468F

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