Issue 17, 2013
From the journal:

Analyst

Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis†

Juanni Zhang,a   Jianniao Tian,*a   Yanlong He,a   Sheng Chen,a   Yixuan Jiang,a   Yanchun Zhao*a  and  Shulin Zhaoa  

* Corresponding authors

a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, China
E-mail: birdtjn@sina.com
Fax: +86-7735832294
Tel: +86-7735846279

Abstract

We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.

Graphical abstract: Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis

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Article information

DOI
https://doi.org/10.1039/C3AN00830D
Article type
Communication
Submitted
26 Apr 2013
Accepted
08 Jun 2013
First published
10 Jun 2013

Analyst, 2013,138, 4722-4727

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Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis

J. Zhang, J. Tian, Y. He, S. Chen, Y. Jiang, Y. Zhao and S. Zhao, Analyst, 2013, 138, 4722 DOI: 10.1039/C3AN00830D

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