Issue 18, 2013

Aptamer-linked biosensor for thrombin based on AuNPs/thionine–graphene nanocomposite

Abstract

A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine–graphene (AuNPs/Th–G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine via π-stacking interaction, and then AuNPs were electrodeposited onto the Th–G surface. The morphology and conductivity of the AuNPs/Th–G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th–G nanocomposite to prepare an electrochemical biosensing platform. The aptamer–thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).

Graphical abstract: Aptamer-linked biosensor for thrombin based on AuNPs/thionine–graphene nanocomposite

Article information

Article type
Paper
Submitted
20 May 2013
Accepted
19 Jun 2013
First published
20 Jun 2013

Analyst, 2013,138, 5365-5370

Aptamer-linked biosensor for thrombin based on AuNPs/thioninegraphene nanocomposite

Z. Zhang, L. Luo, L. Zhu, Y. Ding, D. Deng and Z. Wang, Analyst, 2013, 138, 5365 DOI: 10.1039/C3AN01006F

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