Issue 24, 2013

Raman database of amino acids solutions: a critical study of Extended Multiplicative Signal Correction

Abstract

The Raman spectra of biological materials always exhibit complex profiles, constituting several peaks and/or bands which arise due to the large variety of biomolecules. The extraction of quantitative information from these spectra is not a trivial task. While qualitative information can be retrieved from the changes in peaks frequencies or from the appearance/disappearance of some peaks, quantitative analysis requires an examination of peak intensities. Unfortunately in biological samples it is not easy to identify a reference peak for normalizing intensities, and this makes it very difficult to study the peak intensities. In the last decades a more refined mathematical tool, the extended multiplicative signal correction (EMSC), has been proposed for treating infrared spectra, which is also capable of providing quantitative information. From the mathematical and physical point of view, EMSC can also be applied to Raman spectra, as recently proposed. In this work the reliability of the EMSC procedure is tested by application to a well defined biological system: the 20 standard amino acids and their combination in peptides. The first step is the collection of a Raman database of these 20 amino acids, and subsequently EMSC processing is applied to retrieve quantitative information from amino acids mixtures and peptides. A critical review of the results is presented, showing that EMSC has to be carefully handled for complex biological systems.

Graphical abstract: Raman database of amino acids solutions: a critical study of Extended Multiplicative Signal Correction

Article information

Article type
Paper
Submitted
03 Sep 2013
Accepted
02 Oct 2013
First published
02 Oct 2013

Analyst, 2013,138, 7331-7340

Raman database of amino acids solutions: a critical study of Extended Multiplicative Signal Correction

P. Candeloro, E. Grande, R. Raimondo, D. Di Mascolo, F. Gentile, M. L. Coluccio, G. Perozziello, N. Malara, M. Francardi and E. Di Fabrizio, Analyst, 2013, 138, 7331 DOI: 10.1039/C3AN01665J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements