Issue 2, 2014

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

Abstract

The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.

Graphical abstract: Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

Article information

Article type
Paper
Submitted
26 Sep 2013
Accepted
31 Oct 2013
First published
31 Oct 2013

Analyst, 2014,139, 439-445

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

S. Zhang, A. Garcia-D'Angeli, J. P. Brennan and Q. Huo, Analyst, 2014, 139, 439 DOI: 10.1039/C3AN01835K

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