Issue 8, 2013
From the journal:

Analyst

A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules†

Xinggui Gu,a   Guanxin Zhang,*a   Zhuo Wang,b   Wenwen Liu,b   Le Xiaob  and  Deqing Zhang*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
E-mail: dqzhang@iccas.ac.cn

b Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing 100080, China

Abstract

Based on the consideration that compound 1 with a –PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL−1 can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP.

Graphical abstract: A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C3AN36784C
Article type
Paper
Submitted
01 Dec 2012
Accepted
05 Feb 2013
First published
05 Feb 2013

Analyst, 2013,138, 2427-2431

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A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules

X. Gu, G. Zhang, Z. Wang, W. Liu, L. Xiao and D. Zhang, Analyst, 2013, 138, 2427 DOI: 10.1039/C3AN36784C

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