Issue 6, 2013

Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform

Abstract

A simple and sensitive label-free electrochemiluminescence (ECL) immunosensor based on the use of luminol functionalized gold nanoparticles (luminol-AuNPs) as antibody carriers and sensing platform is described for detecting the acute myocardial infarction biomarker cTnI. The ECL immunosensor was fabricated by the assembly of luminol-AuNPs conjugated with biotinylated antibodies against cTnI (biotin-anti-cTnI-luminol-AuNPs) with the streptavidin coated AuNPs (SA-AuNPs) modified Au electrode directly by virtue of the biotin-SA system. The fabricated sensing platform exhibited stable and strong ECL intensity and could be used for the recognition of target antigen. In the presence of cTnI, a decrease in the ECL intensity was observed. Direct detection of the ECL signal changes during antigen–antibody immunoreactions can be used for the quantification of cTnI. The ECL response exhibited a quite wide dynamic range from 1000 ng mL−1 down to 0.1 ng mL−1. The proposed method has been successfully applied in the detection of cTnI in real plasma samples. This protocol is simple, fast, sensitive, specific, stable and reliable. This work reveals that the luminol-AuNPs are excellent sensing platforms for the fabrication of simple and sensitive immunosensors. Moreover, the proposed strategy may also be extended for the detection of other biomarkers, which is of great application potential in clinical and pharmaceutical analysis.

Graphical abstract: Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform

Supplementary files

Article information

Article type
Paper
Submitted
04 Dec 2012
Accepted
09 Jan 2013
First published
09 Jan 2013

Analyst, 2013,138, 1844-1850

Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform

F. Li, Y. Yu, H. Cui, D. Yang and Z. Bian, Analyst, 2013, 138, 1844 DOI: 10.1039/C3AN36805J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements