Issue 24, 2013

Quantitative determination of platinum derived from cisplatin in human plasma ultrafiltrate using derivatization with diethyldithiocarbamate and liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Abstract

This paper describes a method for the determination of platinum derived from cisplatin in human plasma ultrafiltrate using liquid chromatography tandem mass spectrometry (LC-MS/MS). Cisplatin and the internal standard trans-diamminedichloropalladium(II) (TDPD) were chelated with diethyldithiocarbamate (DDTC) and separated with an isocratic elution on a C18 column. The method was validated with intra-/inter-batch accuracy and precision (relative standard deviation, RSD, %), matrix effect, specificity, limit of quantification (LOQ), linearity and stability. The linear range was 1.0–100.0 ng mL−1 and the LOQ was 1.0 ng mL−1. The intra-/inter-batch accuracy and precision were 92.2–111.0%/94.7–106.0% and 3.9–8.8%/2.1–9.3%, respectively. This sensitive and interference-free method can permit reliable and accurate analysis of platinum derived from cisplatin in human plasma ultrafiltrate.

Graphical abstract: Quantitative determination of platinum derived from cisplatin in human plasma ultrafiltrate using derivatization with diethyldithiocarbamate and liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Article information

Article type
Paper
Submitted
22 Sep 2013
Accepted
12 Oct 2013
First published
15 Oct 2013

Anal. Methods, 2013,5, 7117-7126

Quantitative determination of platinum derived from cisplatin in human plasma ultrafiltrate using derivatization with diethyldithiocarbamate and liquid chromatography coupled with electrospray ionization tandem mass spectrometry

C. Tang, C. Li, C. Tang, W. Zhan, H. Zheng and X. Peng, Anal. Methods, 2013, 5, 7117 DOI: 10.1039/C3AY41649F

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