Issue 55, 2013

Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor

Abstract

A general approach to mimic the sensing scheme of allosteric enzymes is developed. Through the covalent labeling of a sulfonamide inhibitor to the enzyme HCAII via SNAP-tag protein, the enzyme is rendered inactive. Catalytic activation is triggered only when a protease is present to cleave the recognized peptide sequence.

Graphical abstract: Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor

Supplementary files

Article information

Article type
Communication
Submitted
16 Apr 2013
Accepted
21 May 2013
First published
22 May 2013

Chem. Commun., 2013,49, 6212-6214

Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor

P. Shih, T. Liu and K. Tan, Chem. Commun., 2013, 49, 6212 DOI: 10.1039/C3CC42791A

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