Issue 86, 2013

5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

Abstract

Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by the exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, we first conducted a comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, 5-methyldeoxycytidine could enhance the substrate activity of the Klenow fragment (exo-) and this feature could potentially be used in DNA methylation analysis.

Graphical abstract: 5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

Supplementary files

Article information

Article type
Communication
Submitted
28 May 2013
Accepted
05 Sep 2013
First published
05 Sep 2013

Chem. Commun., 2013,49, 10085-10087

5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

T. Tian, S. Peng, H. Xiao, Y. Long, B. Fu, X. Zhang, S. Guo, S. Wang, X. Zhou, S. Liu and X. Zhou, Chem. Commun., 2013, 49, 10085 DOI: 10.1039/C3CC44017F

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