Issue 85, 2013
From the journal:

Chemical Communications

Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using ribonucleotide-modified DNA probes†

Pengbo Zhang,a   Ye Liu,a   Yajing Zhang,a   Chenghui Liu,*a   Zhibin Wanga  and  Zhengping Li*a  

* Corresponding authors

a Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China
E-mail: liuch@hbu.edu.cn, lzpbd@hbu.edu.cn
Fax: +86 312 5079403

Abstract

Using the ribonucleotide-modified DNA probes, multiple microRNAs can be simultaneously detected in one ligation-based PCR reaction. As low as 0.2 fM microRNA can be accurately detected with high specificity.

Graphical abstract: Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using ribonucleotide-modified DNA probes

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Article information

DOI
https://doi.org/10.1039/C3CC45760E
Article type
Communication
Submitted
28 Jul 2013
Accepted
03 Sep 2013
First published
05 Sep 2013

Chem. Commun., 2013,49, 10013-10015

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Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using ribonucleotide-modified DNA probes

P. Zhang, Y. Liu, Y. Zhang, C. Liu, Z. Wang and Z. Li, Chem. Commun., 2013, 49, 10013 DOI: 10.1039/C3CC45760E

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