Issue 11, 2014
From the journal:

Chemical Communications

A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry†

Ayan Samanta,‡a   André Krause‡a  and  Andres Jäschke*a  

* Corresponding authors

a Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany
E-mail: jaeschke@uni-hd.de
Fax: +49 6221 54 6430
Tel: +49 6221 54 4851

Abstract

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one.

Graphical abstract: A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

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Article information

DOI
https://doi.org/10.1039/C3CC46132G
Article type
Communication
Submitted
11 Aug 2013
Accepted
16 Oct 2013
First published
17 Oct 2013

Chem. Commun., 2014,50, 1313-1316

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A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

A. Samanta, A. Krause and A. Jäschke, Chem. Commun., 2014, 50, 1313 DOI: 10.1039/C3CC46132G

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