A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry

Xiaowen Yan; Zhaoxin Li; Yong Liang; Limin Yang; Bo Zhang; Qiuquan Wang

doi:10.1039/C3CC48460B

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A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry†

Xiaowen Yan,^a Zhaoxin Li,^a Yong Liang,^a Limin Yang,^a Bo Zhang^a and Qiuquan Wang*^ab

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* Corresponding authors

^a Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
E-mail: qqwang@xmu.edu.cn
Fax: +86(0)599-2187400

^b State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China

Abstract

A novel element-tagged activity-based photo-cleavable biotinylated chemical “Hub” was designed and synthesized to orthogonally integrate ICP-MS and ESI-MS for absolute targeted-protein quantification. This tetrafunctional chemical “hub” allowed us to quantify a targeted protein using species-unspecific isotope dilution ICP-MS and to know which protein is being quantified using ESI-IT-MS at the same time.

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Article information

DOI: https://doi.org/10.1039/C3CC48460B
Article type: Communication
Submitted: 05 Nov 2013
Accepted: 05 May 2014
First published: 05 May 2014
This article is Open Access

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Chem. Commun., 2014,50, 6578-6581

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A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry

X. Yan, Z. Li, Y. Liang, L. Yang, B. Zhang and Q. Wang, Chem. Commun., 2014, 50, 6578 DOI: 10.1039/C3CC48460B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry†

Abstract

Supplementary files

Article information

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A chemical “hub” for absolute quantification of a targeted protein: orthogonal integration of elemental and molecular mass spectrometry

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