Issue 13, 2014
From the journal:

Chemical Communications

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection†

Liang Cui,a   Zhi Zhu,*a   Ninghang Lin,a   Huimin Zhang,a   Zhichao Guana  and  Chaoyong James Yang*a  

* Corresponding authors

a State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China
E-mail: zhuzhi@xmu.edu.cn, cyyang@xmu.edu.cn

Abstract

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

Graphical abstract: A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

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Article information

DOI
https://doi.org/10.1039/C3CC48707E
Article type
Communication
Submitted
14 Nov 2013
Accepted
05 Dec 2013
First published
05 Dec 2013

Chem. Commun., 2014,50, 1576-1578

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A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

L. Cui, Z. Zhu, N. Lin, H. Zhang, Z. Guan and C. J. Yang, Chem. Commun., 2014, 50, 1576 DOI: 10.1039/C3CC48707E

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