Issue 10, 2013

Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

Abstract

Pulsed electron–electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.

Graphical abstract: Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

Supplementary files

Article information

Article type
Communication
Submitted
08 Dec 2012
Accepted
15 Jan 2013
First published
17 Jan 2013

Phys. Chem. Chem. Phys., 2013,15, 3433-3437

Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

I. Tkach, S. Pornsuwan, C. Höbartner, F. Wachowius, S. Th. Sigurdsson, T. Y. Baranova, U. Diederichsen, G. Sicoli and M. Bennati, Phys. Chem. Chem. Phys., 2013, 15, 3433 DOI: 10.1039/C3CP44415E

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