Issue 4, 2014

Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions

Abstract

Single molecule studies of protein–nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed “protein induced fluorescence enhancement” (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.

Graphical abstract: Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions

Article information

Article type
Review Article
Submitted
15 Jun 2013
First published
23 Sep 2013

Chem. Soc. Rev., 2014,43, 1221-1229

Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions

H. Hwang and S. Myong, Chem. Soc. Rev., 2014, 43, 1221 DOI: 10.1039/C3CS60201J

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