Issue 13, 2014

Mesoporous silica nanoparticles for enhancing the delivery efficiency of immunostimulatory DNA drugs

Abstract

We developed a potential immunostimulatory double-stranded DNA (dsDNA) delivery system by the binding of dsDNA to amino-modified mesoporous silica nanoparticles (MSNs) to form MSN-NH2/dsDNA complexes. Serum stability, in vitro cytotoxicity, cell uptake, and type I interferon-α (IFN-α) induction of MSN-NH2/dsDNA complexes were evaluated. The results showed that MSN-NH2 nanoparticles had no cytotoxicity to Raw 264.7 cells, and MSN-NH2/dsDNA complexes enhanced the serum stability of dsDNA due to the protection by nanoparticles and exhibited a high efficiency of cell uptake due to a small particle size and excellent dispersity. Most importantly, MSN-NH2/dsDNA complexes significantly enhanced the level of IFN-α induction, triggered by cytosolic DNA sensor proteins. Therefore, binding of immunostimulatory DNA to MSNs would play a promising role for enhancing the delivery efficiency of immunostimulatory DNA drugs.

Graphical abstract: Mesoporous silica nanoparticles for enhancing the delivery efficiency of immunostimulatory DNA drugs

Supplementary files

Article information

Article type
Paper
Submitted
06 Dec 2013
Accepted
13 Jan 2014
First published
14 Jan 2014

Dalton Trans., 2014,43, 5142-5150

Author version available

Mesoporous silica nanoparticles for enhancing the delivery efficiency of immunostimulatory DNA drugs

C. Tao, Y. Zhu, Y. Xu, M. Zhu, H. Morita and N. Hanagata, Dalton Trans., 2014, 43, 5142 DOI: 10.1039/C3DT53433B

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