Issue 14, 2013

Molecular-level dengue fever diagnostic devices made out of paper

Abstract

This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.

Graphical abstract: Molecular-level dengue fever diagnostic devices made out of paper

Supplementary files

Article information

Article type
Paper
Submitted
30 Jan 2013
Accepted
12 Mar 2013
First published
12 Mar 2013

Lab Chip, 2013,13, 2686-2692

Molecular-level dengue fever diagnostic devices made out of paper

S. Lo, S. Yang, D. Yao, J. Chen, W. Tu and C. Cheng, Lab Chip, 2013, 13, 2686 DOI: 10.1039/C3LC50135C

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