Issue 14, 2013
From the journal:

Lab on a Chip

Highly sensitive fluorescent protein FRET detection using optofluidic lasers

Qiushu Chen,†a   Xingwang Zhang,†ab   Yuze Sun,a   Michael Ritt,c   Sivaraj Sivaramakrishnanac  and  Xudong Fan*a  

* Corresponding authors

a Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA
E-mail: xsfan@umich.edu

b Key Laboratory for Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, P. R. China

c Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place, Ann Arbor, MI 48109, USA

Abstract

We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein–protein interactions and protein–drug interactions.

Graphical abstract: Highly sensitive fluorescent protein FRET detection using optofluidic lasers

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Article information

DOI
https://doi.org/10.1039/C3LC50207D
Article type
Communication
Submitted
15 Feb 2013
Accepted
13 Mar 2013
First published
13 Mar 2013

Lab Chip, 2013,13, 2679-2681

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Highly sensitive fluorescent protein FRET detection using optofluidic lasers

Q. Chen, X. Zhang, Y. Sun, M. Ritt, S. Sivaramakrishnan and X. Fan, Lab Chip, 2013, 13, 2679 DOI: 10.1039/C3LC50207D

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