Issue 17, 2013

Design of a prototype flow microreactor for synthetic biology in vitro

Abstract

As a reference platform for in vitro synthetic biology, we have developed a prototype flow microreactor for enzymatic biosynthesis. We report the design, implementation, and computer-aided optimisation of a three-step model pathway within a microfluidic reactor. A packed bed format was shown to be optimal for enzyme compartmentalisation after experimental evaluation of several approaches. The specific substrate conversion efficiency could significantly be improved by an optimised parameter set obtained by computational modelling. Our microreactor design provides a platform to explore new in vitro synthetic biology solutions for industrial biosynthesis.

Graphical abstract: Design of a prototype flow microreactor for synthetic biology in vitro

Supplementary files

Article information

Article type
Paper
Submitted
19 Feb 2013
Accepted
27 Jun 2013
First published
27 Jun 2013

Lab Chip, 2013,13, 3426-3432

Design of a prototype flow microreactor for synthetic biology in vitro

C. R. Boehm, P. S. Freemont and O. Ces, Lab Chip, 2013, 13, 3426 DOI: 10.1039/C3LC50231G

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