Issue 19, 2013

Photoelectrochemical lab-on-paper device equipped with a porous Au-paper electrode and fluidic delay-switch for sensitive detection of DNA hybridization

Abstract

The sequence-specific detection of DNA hybridization has attracted considerable interest in numerous fields. Although traditional DNA biosensors have been widely explored due to their high sensitivity, it is still challenging to develop a low-cost, portable, disposable, fast, and easy-to-use DNA detection method for public use at home or in the field. To address these challenges, herein, we report a novel microfluidic photoelectrochemical (PEC) paper-based analytical platform, integrated with an internal chemiluminescent light source, a novel paper supercapacitor (PS) amplifier, and a terminal digital multi-meter (DMM) detector, for sensitive DNA detection using a graphene-modified porous Au-paper electrode as the working electrode to obtain enhanced PEC responses. The quantification mechanism of this strategy is based on the charging of this PS, which was constructed on a paper-based analytical platform through a simple “drawing and soaking” method, by the generated photocurrent. After a fixed period, the PS was automatically shorted under the control of a novel built-in fluidic delay-switch to output an instantaneously amplified current, which could be sensitively detected by the DMM. At optimal conditions, this paper-based analytical platform can detect DNA at concentrations at femtomolar level. This approach also shows excellent specificity toward single nucleotide mismatches.

Graphical abstract: Photoelectrochemical lab-on-paper device equipped with a porous Au-paper electrode and fluidic delay-switch for sensitive detection of DNA hybridization

Supplementary files

Article information

Article type
Paper
Submitted
06 Apr 2013
Accepted
19 Jul 2013
First published
25 Jul 2013

Lab Chip, 2013,13, 3945-3955

Photoelectrochemical lab-on-paper device equipped with a porous Au-paper electrode and fluidic delay-switch for sensitive detection of DNA hybridization

Y. Wang, L. Ge, P. Wang, M. Yan, S. Ge, N. Li, J. Yu and J. Huang, Lab Chip, 2013, 13, 3945 DOI: 10.1039/C3LC50430A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements