Issue 15, 2014

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

Abstract

We microfluidically fabricate bio-orthogonal DNA-functionalized porous hydrogels from hyaluronic acid that are employed in in vitro transcription/translation (IVTT) of a green fluorescent protein. By co-encapsulating individual hydrogel particles and the IVTT machinery in water-in-oil microdroplets, we study protein expression in a defined reaction volume. Our approach enables precise control over protein expression rates by gene dosage. We show that gene transcription and translation are confined to the membrane-free hydrogel matrix, which contributes to the design of membrane-free protocells.

Graphical abstract: DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

Supplementary files

Article information

Article type
Paper
Submitted
21 Dec 2013
Accepted
17 Feb 2014
First published
24 Mar 2014

Lab Chip, 2014,14, 2651-2656

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

J. Thiele, Y. Ma, D. Foschepoth, M. M. K. Hansen, C. Steffen, H. A. Heus and W. T. S. Huck, Lab Chip, 2014, 14, 2651 DOI: 10.1039/C3LC51427G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements