Issue 4, 2013
From the journal:

Molecular BioSystems

Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization†

Pil Seok Chae,*a   Marc J. Wander,b   Kyung Ho Cho,a   Philip D. Laibleb  and  Samuel H. Gellman*c  

* Corresponding authors

a Department of Bionano Engineering, Hanyang University, Ansan, Korea
E-mail: pchae@hanyang.ac.kr

b Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
E-mail: laible@anl.gov

c Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
E-mail: gellman@chem.wisc.edu

Abstract

Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.

Graphical abstract: Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

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DOI
https://doi.org/10.1039/C3MB25584K
Article type
Communication
Submitted
18 Dec 2012
Accepted
23 Jan 2013
First published
24 Jan 2013

Mol. BioSyst., 2013,9, 626-629

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Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

P. S. Chae, M. J. Wander, K. H. Cho, P. D. Laible and S. H. Gellman, Mol. BioSyst., 2013, 9, 626 DOI: 10.1039/C3MB25584K

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