Issue 10, 2013
From the journal:

New Journal of Chemistry

An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems

Ludivine Garcia,a   Mirca Lazzaretti,b   Antoine Diguet,cde   Francesca Mussi,b   Franco Bisceglie,f   Juan Xie,g   Giorgio Pelosi,f   Annamaria Buschini,b   Damien Baiglcde  and  Clotilde Policar*cdh  

* Corresponding authors

a Univ. Paris-Sud 11, ICMMO, Equipe de Chimie Bioorganique et Bioinorganique, F-91405 Orsay Cedex, France

b Università degli Studi di Parma, Dipartimento di Bioscienze, 43124 Parma, Italy

c Department of Chemistry, Ecole Normale Supérieure, 24, rue Lhomond, 75005 Paris, France
E-mail: clotilde.policar@ens.fr
Fax: +33 1 44 32 33 97
Tel: +33 1 44 32 24 20

d Université Pierre et Marie Curie Paris 6, 4 Place Jussieu, 75005 Paris, France

e Laboratoire PASTEUR UMR 8640, CNRS, France

f Universita degli Studi di Parma, Dipartimento di Chimica, 43124 Parma, Italy

g PPSM, Institut d'Alembert, ENS Cachan, CNRS, 61 av Président Wilson, F-94230, Cachan, France

h Laboratoire des BioMolécules, UMR7203, CNRS, France

Abstract

Glycoligands, sugar-based molecules able to complex metal cations, constitute a new class of molecules with great potential for biological and biochemical applications. To analyze their behaviour in a biological environment, we have synthesized an intrinsically fluorescent glycoligand and analyzed its trafficking in both living (U937 human cancer cells) and artificial (giant unilamellar vesicles) cell systems. We have found that this ligand has moderate cytotoxicity accompanied by specific accumulation in both living and reconstituted membranes, which it can cross to reach inner compartments.

Graphical abstract: An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems

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Article information

DOI
https://doi.org/10.1039/C3NJ00380A
Article type
Paper
Submitted
10 Apr 2013
Accepted
08 Jul 2013
First published
08 Jul 2013

New J. Chem., 2013,37, 3030-3034

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An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems

L. Garcia, M. Lazzaretti, A. Diguet, F. Mussi, F. Bisceglie, J. Xie, G. Pelosi, A. Buschini, D. Baigl and C. Policar, New J. Chem., 2013, 37, 3030 DOI: 10.1039/C3NJ00380A

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