Issue 7, 2013

Colorimetric detection of glutathione in human blood serum based on the reduction of oxidized TMB

Abstract

In this paper, a novel and simple colorimetric method for the determination of glutathione (GSH) was developed. The BSA–MnO2 NPs possess oxidase-like activity that can catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB), and the existence of GSH can cause reduction of oxidized TMB along with a visual color variation. A good linear relationship can be obtained from 0.26 to 26 μM with a limit of detection of 0.1 μM. Furthermore, the good recoveries for serum samples indicated that the present colorimetric methodology was feasible, simple and sensitive, with a promising application in routine analysis of biosamples.

Graphical abstract: Colorimetric detection of glutathione in human blood serum based on the reduction of oxidized TMB

Article information

Article type
Paper
Submitted
07 Oct 2012
Accepted
17 Apr 2013
First published
18 Apr 2013

New J. Chem., 2013,37, 2174-2178

Colorimetric detection of glutathione in human blood serum based on the reduction of oxidized TMB

X. Liu, Q. Wang, Y. Zhang, L. Zhang, Y. Su and Y. Lv, New J. Chem., 2013, 37, 2174 DOI: 10.1039/C3NJ40897C

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