Issue 19, 2013
From the journal:

Nanoscale

Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity†

Peng Chen,ab   Robert Selegård,c   Daniel Ailic  and  Bo Liedberg*abc  

* Corresponding authors

a Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore
E-mail: bliedberg@ntu.edu.sg

b School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore

c Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Sweden

Abstract

A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL−1).

Graphical abstract: Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

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Article information

DOI
https://doi.org/10.1039/C3NR03006G
Article type
Communication
Submitted
11 Jun 2013
Accepted
01 Aug 2013
First published
08 Aug 2013

Nanoscale, 2013,5, 8973-8976

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Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

P. Chen, R. Selegård, D. Aili and B. Liedberg, Nanoscale, 2013, 5, 8973 DOI: 10.1039/C3NR03006G

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