Issue 4, 2014
From the journal:

Organic & Biomolecular Chemistry

Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence ‘turn-on’ reagents†

Judith Youziel,a   Ahmed R. Akhbar,a   Qadeer Aziz,b   Mark E. B. Smith,a   Stephen Caddick,a   Andrew Tinkerb  and  James R. Baker*a  

* Corresponding authors

a Department of Chemistry, University College London, 20 Gordon St, London, UK
E-mail: j.r.baker@ucl.ac.uk
Tel: +44 (0)2076792653

b Barts and The London, Queen Mary's School of Medicine and Dentistry, Charterhouse Square, London, UK

Abstract

Bromo- and thiomaleimides are shown to serve as highly effective quenchers of a covalently attached fluorophore. Reactions with thiols that lead to removal of the maleimide conjugation, or detachment of the fluorophore from the maleimide, result in ‘turn-on’ of the fluorescence. These reagents thus offer opportunities in thiol sensing and intracellular reporting.

Graphical abstract: Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence ‘turn-on’ reagents

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Article information

DOI
https://doi.org/10.1039/C3OB42141D
Article type
Communication
Submitted
28 Oct 2013
Accepted
25 Nov 2013
First published
25 Nov 2013
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2014,12, 557-560

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Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence ‘turn-on’ reagents

J. Youziel, A. R. Akhbar, Q. Aziz, M. E. B. Smith, S. Caddick, A. Tinker and J. R. Baker, Org. Biomol. Chem., 2014, 12, 557 DOI: 10.1039/C3OB42141D

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