Amphiphilic nanogel of enzymatically synthesized glycogen as an artificial molecular chaperone for effective protein refolding

Abstract

Enzymatically synthesized glycogen (ESG) bearing hydrophobic groups were prepared to construct a new artificial molecular chaperone system. The radius of the amphiphilic ESG nanogel was approximately 15 nm. The nanogels prevented irreversible aggregation of carbonic anhydrase. Cyclodextrin induced the release of carbonic anhydrase in its active form via a one-step mechanism without dissociation of the nanogels.