Issue 45, 2013

Monolayer spontaneous curvature of raft-forming membrane lipids

Abstract

Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (HII). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a HII forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J0 values are closer to zero. Interestingly, a significant positive J0 value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J0(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10−3 (nm °C)−1 for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension.

Graphical abstract: Monolayer spontaneous curvature of raft-forming membrane lipids

Supplementary files

Article information

Article type
Paper
Submitted
04 Jul 2013
Accepted
24 Sep 2013
First published
27 Sep 2013
This article is Open Access
Creative Commons BY-NC license

Soft Matter, 2013,9, 10877-10884

Monolayer spontaneous curvature of raft-forming membrane lipids

B. Kollmitzer, P. Heftberger, M. Rappolt and G. Pabst, Soft Matter, 2013, 9, 10877 DOI: 10.1039/C3SM51829A

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