Issue 35, 2013

High-throughput nanoprecipitation of the organic antimicrobial triclosan and enhancement of activity against Escherichia coli

Abstract

Enhancing the activity of existing antimicrobial agents may help to address the emergence of resistant bacteria. Nanoparticles of antimicrobial agents have previously been shown to provide potential activity enhancements and here we report a high-throughput nanoprecipitation approach to identify viable nanosuspensions of the antimicrobial compound triclosan. Through careful choice of the components of the nanoprecipitation, amorphous nanosuspensions were created, freeze-dried and redispersed in water with z-average diameters varying from 170–290 nm. Particle size was shown to be controlled by a series of factors including polymer/surfactant concentration and concentration of triclosan solution prior to nanoprecipitation. A ten-fold decrease (i.e. higher activity) in the Escherichia coli (E. coli) inhibitory concentration (IC50) of triclosan, compared to an aqueous control, was observed for nanoparticles prepared using Pluronic® F68 and the cationic surfactant Hyamine. This overall approach offers a rapid route for identifying viable nanosuspensions and enhancing the properties of commercially available biologically active compounds with poor water-solubility.

Graphical abstract: High-throughput nanoprecipitation of the organic antimicrobial triclosan and enhancement of activity against Escherichia coli

Supplementary files

Article information

Article type
Paper
Submitted
16 Apr 2013
Accepted
19 Jul 2013
First published
22 Jul 2013

J. Mater. Chem. B, 2013,1, 4455-4465

High-throughput nanoprecipitation of the organic antimicrobial triclosan and enhancement of activity against Escherichia coli

T. O. McDonald, L. M. Tatham, F. Y. Southworth, M. Giardiello, P. Martin, N. J. Liptrott, A. Owen and S. P. Rannard, J. Mater. Chem. B, 2013, 1, 4455 DOI: 10.1039/C3TB20543F

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