Issue 40, 2013
From the journal:

Journal of Materials Chemistry B

Fluorene-derived two-photonfluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization†

Hua Zhang,a   Jiangli Fan,*a   Huijuan Dong,a   Shuangzhe Zhang,a   Wenyou Xu,a   Jingyun Wang,b   Pan Gaob  and  Xiaojun Peng*a  

* Corresponding authors

a State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, P.R. China
E-mail: fanjl@dlut.edu.cn, pengxj@dlut.edu.cn

b School of Life Science and Biotechnology, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, P.R. China

Abstract

Two novel fluorene-derived two-photon fluorescent probes (TPFL) targeting the endoplasmic reticulum (TPFL-ER) and lysosomes (TPFL-Lyso) were synthesized by introducing a chlorine group and a morpholine group, respectively. They were shown to be suitable for specific and simultaneous imaging of the endoplasmic reticulum and lysosomes without affecting protein movements owing to their similar molecular structures. TPFL-ER and TPFL-Lyso were successfully used to visualize the changes of the endoplasmic reticulum and lysosomes during cancer cell apoptosis and they demonstrated high specificity and sensitivity, excellent photostability, and low phototoxicity.

Graphical abstract: Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization

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Article information

DOI
https://doi.org/10.1039/C3TB20646G
Article type
Paper
Submitted
04 May 2013
Accepted
08 Aug 2013
First published
08 Aug 2013

J. Mater. Chem. B, 2013,1, 5450-5455

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Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization

H. Zhang, J. Fan, H. Dong, S. Zhang, W. Xu, J. Wang, P. Gao and X. Peng, J. Mater. Chem. B, 2013, 1, 5450 DOI: 10.1039/C3TB20646G

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