Issue 12, 2014

A label-free fluorescence detection strategy for lysozyme assay using CuInS2 quantum dots

Abstract

We have developed a simple and efficient fluorescence detection system for lysozyme utilizing I–III–VI type Cu–In–S ternary quantum dots (CuInS2 QDs) as the probe. Water-soluble near-infrared CuInS2 QDs capped with 3-mercaptopropionic acid were directly synthesized by using a hydrothermal method. Poly(dimethyl diallyl ammonium chloride), as a cationic polyelectrolyte, could bind to 3-mercaptopropionic acid-capped CuInS2 QDs via electrostatic interactions that would lead to fluorescence quenching of CuInS2 QDs. And the addition of a lysozyme aptamer could effectively turn on the quenched fluorescence due to the stronger interaction between the cationic polyelectrolyte and negatively charged aptamer. The addition of lysozyme would result in fluorescence quenching of CuInS2 QDs again due to the specific binding between lysozyme and the lysozyme aptamer. Therefore, this label-free fluorescence system could be applied to selectively detect lysozyme.

Graphical abstract: A label-free fluorescence detection strategy for lysozyme assay using CuInS2 quantum dots

Article information

Article type
Paper
Submitted
22 Jan 2014
Accepted
18 Mar 2014
First published
19 Mar 2014

Analyst, 2014,139, 3048-3054

A label-free fluorescence detection strategy for lysozyme assay using CuInS2 quantum dots

S. Liu, W. Na, S. Pang, F. Shi and X. Su, Analyst, 2014, 139, 3048 DOI: 10.1039/C4AN00160E

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