Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle–SWCNT bioconjugated nanohybrids

Abstract

Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs–gold nanoparticle (SWCNT–GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A targeted photothermolysis experiment using 670 nm light at 2 W cm⁻² for 15 min, resulted in selective and irreparable damage to more than 99% Salmonella DT104 at the concentration of 10⁵ CFU mL⁻¹. In comparison to solely SWCNTs or GNPs, our SWCNT–GNPs nanohybrids have also shown a better photothermal efficiency.